Proceedings of MATSUS Fall 2023 Conference (MATSUSFall23)
DOI: https://doi.org/10.29363/nanoge.matsus.2023.144
Publication date: 18th July 2023
Eman Aljawi, Akbota Kurmangaliyeva, Muhammad Jamali, Bushara Fatma, Waqas Waheed, Dean Everett, Charalampos Pitsalidis, Anas Alazzam, and Anna Maria Pappa.
“Bioelectrocatalytic lab on chip device for infection biomarker monitoring”
Viruses can easily mutate, as evidenced with SARS-Cov2, hence prevention and early detection of infection seems to be the most reliable strategies to cope with pandemics. The current available diagnostics tools, such as real-time polymerize chain reaction (RT-PCR), are not compatible with early detection as they are time-consuming, expensive, don’t offer multiplexing, and don’t offer on-site detection. To tackle these issues, Lab-on-Chip (LoC) biosensors have emerged, overcoming the limitations of conventional methods. Electrochemical biosensors mainly consist of electrodes integrated into a platform to offer portable, on-site, cost-effective, specific, and fast detection. This project introduces a multiplexing LoC biosensor for the simultaneous detection of 4 infection biomarkers; glucose, lactate, hydrogen peroxide, and C-reactive protein (CRP) all in one device using biocatalytic enzymes for the three metabolies and an antigen for CRP as biorecognition elements. Optimization on the electrode materials in terms of bioelectrocatalytic performance leads to the optimum sensing capabilities while allowing for multiplexing on a chip. This work compbines LoC technologies based on microfluidics and the recent advancements in materials science, combining conducting polymers with 2D electronic materials for the electrode fabrication.