Publication date: 14th January 2022
Electrophysiological recordings using multi-electrode arrays (MEAs) are well-established. However, a major limitation of state-of-the-art glass-based MEAs is long-term viability and cell survival of brain slice cultures. Since an intact vascular circulation is not preserved after explantation, complex neural tissue requires a defined nutrient and oxygen supply to survive in vitro (del Turco and Deller 2007), which a mesh-style MEA can provide.
The presented MEA for explanted murine or human brain tissue takes advantage of a novel type of conformable, flexible design. 64 IrOx electrodes are distributed along ten 19-µm-wide strings of polyimide (PI) with a thickness of 6 µm, enabling the long-term survival of neural tissue cultures in combination with extracellular electrophysiological recordings.
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