Proceedings of nanoGe Fall Meeting19 (NFM19)
DOI: https://doi.org/10.29363/nanoge.nfm.2019.067
Publication date: 18th July 2019
Although almost 30 years old, surface potential determination by Kevin-probe Force Microscopy (KPFM) is surprisingly little used for investigations of biological processes or materials. This could be due to the fact that KPFM, as an electrical measurement mode of AFM, is not considered to be readily amenable to in-water experiments. However, even KPFM performed in air, when conducted correctly, can tell us a lot about properties of biological samples. Here, I will give an overview of the possibilities, requirements and pitfalls of KPFM on such samples. A particular focus will be on sample preparation, the influence of the most relevant functional surface groups [1], and the influence of air humidity on the KPFM signal [2]. An example will be shown, where KPFM is used to study subtle surface charge shifts of collagen extracellular matrix fibres, which could have significant implications in cell adhesion, bone metabolism and the design of novel tissue scaffolds [3]. There are probably no other analytical methods at present to detect such small but significant changes of the electrical properties of biological materials while maintaining a high, spatial resolution. In addition, a brief overview will be given of current activities and challenges to expand AFM-based surface potential measurements to true in-water experiments [4].