Proceedings of nanoGe September Meeting 2015 (NFM15)
Publication date: 8th June 2015
Quantum dot (QD), with its broad absorption, narrow emission, high quantum yield and exceptional photostability, has drawn a lot of interest in the past several decades for its promising applications in biological imaging researches. Compared to conventional organic fluorophores, QDs have shown advantages in multiple biological applications such as particle tracking and multiplexed imaging. Here, we developed a color series of visible light emitting QDs with nearly unity PL quantum yield, symmetric and narrow emission spectral lineshapes (FWHM 20-25 nm) for highly multiplexed imaging. Additionally, InAs/CdSe core/shell QDs emitting in the short wavelength infrared (SWIR) region were also synthesized to widen the imaging range. To functionalize these QDs for biological use, we developed a novel organic ligand that binds strongly to the surface of colloidal nanocrystallites during QD synthesis and bears a norbornene functional group, which enables efficient conjugation of hydrophilic ligands with tetrazine groups via bioorthogonal click reaction, avoiding the complicated ligand exchange steps.
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