Proceedings of MATSUS Spring 2025 Conference (MATSUSSpring25)
DOI: https://doi.org/10.29363/nanoge.matsusspring.2025.086
Publication date: 16th December 2024
When cells internalized nanoparticles via endocytosis this involves also proteins bound to the surface of the nanoparticles. After internalization, the original protein corona may be partly exchanged. In case the proteins bear labels providing contrast for imaging, the in vivo distribution of the originally bound proteins as well as the one of the nanoparticles can be determined. This can be done for example with fluorescence or X-ray fluorescence based method. Colocalization analysis then provides information about the degree in which the original protein corona is retained.
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