DOI: https://doi.org/10.29363/nanoge.eimc.2021.011
Publication date: 5th July 2021
A minority of cells with unique phenotypic or genotypic characteristics can drive disease progression and response to therapeutic agents. Therefore, single-cell analysis is critical to the accurate characterization of disease states. A major challenge for the development of next-generation devices for the evaluation of individual cells is the integration of all steps of analysis into a single platform. Such integration would greatly reduce the time and cost of diagnosis while increasing accuracy. Major challenges to the realization of this goal include a need for high volumetric throughput, selectivity for the cells of interest, and parallel and sensitive analysis. We discuss methods that leverage electrochemical and electrokinetic processes at arrays of bipolar electrodes to address each of these challenges. Specifically, we will discuss selective capture of cells by dielectrophoresis at these arrays of these wireless electrodes and the integration of biomolecular assays to achieve sensitive and rapid analysis.
This work is supported by NIH NIBIB Early Career Trailblazer Award R21-EB028583.
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