Publication date: 28th June 2024
Introduction: Cardiovascular diseases persist as a significant global health challenge, therefore, for innovative strategies in this area of tissue engineering, this study investigates the blend of bioactive compounds like nanosheets of molybdenum disulfide (MoS2) [1] and the conductive polymer poly(3-hexylthiophene) (P3HT) within double-network (DN) hydrogels tailored for cardiac patch applications that can be activated by light to enhance cellular interactions, self-healing and tissue integration. Results: Using a 3D printer, we created scaffold designs by extruding blends of MoS2(C-P-M) with a coating of P3HT (C-P-M/P3HT). Analysis showed that the incorporation of MoS2 maintained mechanical stiffness comparable to the pure C-P blend, which is critical for structural support in cardiac patches. The later coating of P3HT enables the scaffolds to respond to light stimuli, significantly enhancing the viability and proliferation of Human Umbilical Vein Endothelial Cells (HUVECs) under illuminated conditions, with increases in cell viability observed up to 160%. Discussion: MoS2 nanoparticles contribute to the mechanical stability of the scaffolds while facilitating enhanced cellular responses. The application of P3HT coatings introduces a novel aspect of light responsiveness, allowing for the controlled activation of the scaffolds upon exposure to specific light wavelengths [2]. Conclusions: The combination of MoS2 for structural integrity and P3HT for light-activated functionality within DN hydrogels presents a robust platform for developing bioelectronic interfaces in cardiac tissue engineering. These interfaces not only support mechanical demands but also enable on-demand, light-induced cellular interactions, paving the way for advanced therapeutic strategies in regenerative medicine and tissue engineering.
Beca Doctorado Nacional ANID No. 21201471, ANID-Basal Center of Interventional Medicine for Precision and Advanced Cellular Therapy, IMPACT, Project No. FB210024, Fondecyt Regular No. 1200093.