DOI: https://doi.org/10.29363/nanoge.cybioel.2024.044
Publication date: 28th June 2024
Therapy for peripheral nerve and spinal cord injuries as well as several neurodegenerative diseases remain a big clinical challenge due to poor regeneration of neural tissue in the damaged area. [1]. Within this context, human induced pluripotent stem cells (hiPSC)-derived neuronal networks present an effective in-vitro model for the study of these physiological issues. However, the differentiation and maturation of hiPSCs is a complex and time-consuming process, thereby necessitating techniques for real-time monitoring of neuronal differentiation and maturation with high sensitivity.
Moreover, physical microenvironmental cues, such as electrical signals, have been increasingly recognized as crucial in regulating stem cell behaviour and fate as well as neuron regeneration processes [2]. However, the underlying molecular mechanism demand further investigation [3]. Furthermore, PEDOT: PSS based electrodes have been shown to lower the electrochemical impedance leading to an increased SNR [4] while recording in addition to promoting neurogenic differentiation [5].
In this work, we first designed a microelectrode array and compared the SNR, electrochemical stability, charge storage capacity and charge injection limit of different electrode materials, viz-a-viz, Gold, Gold/PEDOT: PSS and PEDOT: PSS only via Electrochemical Impedance Spectroscopy (EIS), Voltage Transients and Cyclic Voltammetry (CV) measurements. This is followed by comparison of different electrode designs including inter-digitated, castellated and matrix based. The best electrode designs were used to monitor proliferation of (hiPSCs) followed by the differentiation to cortical neurons with the entire process being analysed by correlating the physiological variations during the process to the variation in impedance magnitude and phase at various frequency bands.
Further studies aim to make the in-vitro platform more biomimetic with flexible mesh-design based electrodes to bridge the dimensional gap between the 3D cultures and the monitoring system.
nanoGe is a prestigious brand of successful science conferences that are developed along the year in different areas of the world since 2009. Our worldwide conferences cover cutting-edge materials topics like perovskite solar cells, photovoltaics, optoelectronics, solar fuel conversion, surface science, catalysis and two-dimensional materials, among many others.
Previously nanoGe Spring Meeting (NSM) and nanoGe Fall Meeting (NFM), MATSUS is a multiple symposia conference focused on a broad set of topics of advanced materials preparation, their fundamental properties, and their applications, in fields such as renewable energy, photovoltaics, lighting, semiconductor quantum dots, 2-D materials synthesis, charge carriers dynamics, microscopy and spectroscopy semiconductors fundamentals, etc.
International Conference on Hybrid and Organic Photovoltaics
International Conference on Hybrid and Organic Photovoltaics (HOPV) is celebrated yearly in May. The main topics are the development, function and modeling of materials and devices for hybrid and organic solar cells. The field is now dominated by perovskite solar cells but also other hybrid technologies, as organic solar cells, quantum dot solar cells, and dye-sensitized solar cells and their integration into devices for photoelectrochemical solar fuel production.
Asia-Pacific International Conference on Perovskite, Organic Photovoltaics and Optoelectronics
The main topics of the Asia-Pacific International Conference on Perovskite, Organic Photovoltaics and Optoelectronics (IPEROP) are discussed every year in Asia-Pacific for gathering the recent advances in the fields of material preparation, modeling and fabrication of perovskite and hybrid and organic materials. Photovoltaic devices are analyzed from fundamental physics and materials properties to a broad set of applications. The conference also covers the developments of perovskite optoelectronics, including light-emitting diodes, lasers, optical devices, nanophotonics, nonlinear optical properties, colloidal nanostructures, photophysics and light-matter coupling.
The International Conference on Perovskite Thin Film Photovoltaics Perovskite Photonics and Optoelectronics (NIPHO) is the best place to hear the latest developments in perovskite solar cells as well as on recent advances in the fields of perovskite light-emitting diodes, lasers, optical devices, nanophotonics, nonlinear optical properties, colloidal nanostructures, photophysics and light-matter coupling.