Publication date: 25th July 2016
I will discuss our efforts to improve structured illumination microscopy (SIM) and light-sheet microscopy. SIM doubles the spatial resolution of light microscopy, requiring lower light intensities and acquisition times than other super-resolution techniques. I will present SIM implementations that enable resolution doubling in live volumes > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively ‘instant’ SIM imaging at rates 10-100x faster than other SIM.
The second half of the talk will focus on the development of inverted selective plane illumination microscopy (iSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. I will discuss progress that quadruples the axial resolution of iSPIM by utilizing a second specimen view, thus enabling imaging with isotropic spatial resolution (dual-view iSPIM, or diSPIM). I also describe futher improvements to the spatial resolution of diSPIM, as well as challenges in applying light-sheet technology to thick, scattering samples. Applications of light-sheet microscopy will be presented, including computational methods for untwisting worm embryos.
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