Publication date: 10th April 2014
Biosensors are of relevant interest for many researchers and industries due to their high selectivity. Development of glucose sensors are of interest in the applications such food control, fermentation or clinical analysis (Diabetes mellitus disease) [1]. Typical glucose-biosensors are based in Glucose Oxidase (GOx) immobilized over a conductive substrate. Other enzymes as Glucose Dehydrogenase can be used in this kind of biodevices although the thermal stability of such dehydrogenases is not as good as GOx preparations. We present a biosensor based in GOx immobilized and electropolimerized with thioaniline-functionalized Au nanoparticles (NPs) growing a three-dimensional Thioaniline/Au NPs/GOx network [2].
The performance of these sensors is based predominantly in electrochemical techniques such amperometry, j-V curves, EIS. Here we use these techniques for studying and characterizing the process of charge transfer from the GOx to glucose and the the different contributions to charge accumulation processes in the three-dimensional network that constitutes the biodevice.
Acknowledgements: European Commission, 282970-BioelectroMET.
Bibliography
[1] J.M. Montornes et al. / Bioelectrochemistry Fundamentals, Experimental Techniques and Applications Chapter 5 (2008) 199-217.
[2] O. Yehezkeli et al./ Chemistry A European Journal 15 (2009) 2674-2679.
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