Publication date: 22nd April 2022
The possibility to enhance tissue repair by activating in an spatio-temporal fashion intracellular pathways is a hot topic in regenerative medicine. It is well known that certain signaling pathways can be triggered by a mechanotransduction process, whereby external mechanical stimuli are converted into biochemical signallings. Cadherins are important cell membrane mechanoretransductors that promote cell-cell adhesion through Ca2+ -dependent homophilic interactions. Among others, cadherin mechanotransduction is critical to induce intracellular cascades in order to mediate collective epithelial remodeling during tissue repair. Thus, cadherins are an attractive target to stimulate intracellular pathways implicated in these processes.
In order to do so, we aim to selectively bind magnetic microparticles (MMPs) functionalized with oriented E-cadherin fragments, to the membrane of E-cadherin-expressing cells. Under the remote application of an external magnetic field, the pulling force exerted on the MMPs will be used to trigger mechanotransduction processes and intracellular signallings.